{"product_id":"analytical-chemistry-isbn-9781119894452","title":"Analytical Chemistry","description":"\u003cp\u003e\u003cb\u003eANALYTICAL CHEMISTRY\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eDetailed reference covering all aspects of working in laboratories, including safety, fundamentals of analytical techniques, lab instrumentation, and more\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eA comprehensive study of analytical chemistry as it pertains to the laboratory analyst and chemist, \u003ci\u003eAnalytical Chemistry\u003c\/i\u003e begins with an introduction to the laboratory environment, including safety, glassware, common apparatuses, and lab basics, and continues on to guide readers through the fundamentals of analytical techniques, such as spectroscopy and chromatography, and introduce examples of laboratory programs, such as Laboratory Information Management Systems (LIMS).\u003c\/p\u003e \u003cp\u003eThis newly updated and revised Second Edition of \u003ci\u003eAnalytical Chemistry\u003c\/i\u003e offers expanded chapters with new figures and the latest developments in the field.\u003c\/p\u003e \u003cp\u003eIncluded alongside this new edition is an updated companion teaching, reference, and toolkit program called ChemTech. Conveniently available via either app or browser, the ChemTech program contains exercises that highlight and review topics covered in the book and features useful calculators and programs, including solution makers, graphing tools, and more. To aid in reader comprehension, the program also includes an interactive periodic table and chapter summaries.\u003c\/p\u003e \u003cp\u003eWritten by two highly qualified authors with significant experience in both practice and academia, \u003ci\u003eAnalytical Chemistry\u003c\/i\u003e covers sample topics such as:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eBasic mathematics in the laboratory, including different units, the metric system, significant figures, scientific calculators, and ChemTech conversion tools\u003c\/li\u003e \u003cli\u003eAnalytical data treatment, including errors in the laboratory, precision versus accuracy, normal distribution curves, and determining errors in methodology\u003c\/li\u003e \u003cli\u003ePlotting and graphing, including graph construction, curve fitting, graphs of specific equations, least-squares method, and computer-generated curves\u003c\/li\u003e \u003cli\u003eUltraviolet\/visible (UV\/Vis) spectroscopy, including wave and particle theory of light, light absorption transitions, the color wheel, and pigments\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWith complete coverage of the practical aspects of analytical chemistry, \u003ci\u003eAnalytical Chemistry\u003c\/i\u003e prepares students for a rewarding career as a chemist or a laboratory technician. Thanks to ChemTech integration, the book is also a useful and accessible reference for the established chemist or technician already working in the laboratory.\u003c\/p\u003e \u003cp\u003eAbout the Authors xxiii\u003c\/p\u003e \u003cp\u003ePreface xxv\u003c\/p\u003e \u003cp\u003eAcknowledgments xxvii\u003c\/p\u003e \u003cp\u003eAbout the Companion Website xxviii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Chemist and Technician in the Analytical Laboratory 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction—The Analytical Chemist and Technician 1\u003c\/p\u003e \u003cp\u003e1.2 Today’s Laboratory Chemist and Technician 1\u003c\/p\u003e \u003cp\u003e1.3 ChemTech—The Chemist and Technician Toolkit Companion 2\u003c\/p\u003e \u003cp\u003e1.4 Chapter Layout 2\u003c\/p\u003e \u003cp\u003e1.5 Users of ChemTech 6\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Introduction to the Analytical Laboratory 7\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction to the Laboratory 7\u003c\/p\u003e \u003cp\u003e2.2 Laboratory Glassware 7\u003c\/p\u003e \u003cp\u003e2.3 Conclusion 18\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Laboratory Safety 19\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 19\u003c\/p\u003e \u003cp\u003e3.2 Proper Personal Protection and Appropriate Attire 19\u003c\/p\u003e \u003cp\u003e3.3 Proper Shoes and Pants 20\u003c\/p\u003e \u003cp\u003e3.4 Laboratory Gloves 20\u003c\/p\u003e \u003cp\u003e3.5 General Rules to Use Gloves 22\u003c\/p\u003e \u003cp\u003e3.6 Safety Data Sheet (SDS) 22\u003c\/p\u003e \u003cp\u003e3.7 Emergency Eyewash and Face Wash Stations 24\u003c\/p\u003e \u003cp\u003e3.8 Emergency Safety Showers 25\u003c\/p\u003e \u003cp\u003e3.9 Fire Extinguishers 25\u003c\/p\u003e \u003cp\u003e3.10 Clothing Fire in The Laboratory 26\u003c\/p\u003e \u003cp\u003e3.11 Spill Cleanup Kits 26\u003c\/p\u003e \u003cp\u003e3.12 Chemicals and Solvents 27\u003c\/p\u003e \u003cp\u003e3.13 First Aid Kits 29\u003c\/p\u003e \u003cp\u003e3.14 Gasses and Cylinders 29\u003c\/p\u003e \u003cp\u003e3.15 Sharps Containers and Broken Glass Boxes 30\u003c\/p\u003e \u003cp\u003e3.16 Occupational Safety and Health Administration (OSHA) 30\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Basic Mathematics in the Laboratory 83\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction to Basic Math 83\u003c\/p\u003e \u003cp\u003e4.2 Units and Metric System 83\u003c\/p\u003e \u003cp\u003e4.3 Significant Figures 84\u003c\/p\u003e \u003cp\u003e4.4 Scientific Calculators 86\u003c\/p\u003e \u003cp\u003e4.5 ChemTech Conversion Tool 89\u003c\/p\u003e \u003cp\u003e4.6 Chapter Key Concepts 96\u003c\/p\u003e \u003cp\u003e4.7 Chapter Problems 96\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Analytical Data Treatment (Statistics) 97\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Errors in the Laboratory 97\u003c\/p\u003e \u003cp\u003e5.2 Expressing Absolute and Relative Errors 98\u003c\/p\u003e \u003cp\u003e5.3 Precision 98\u003c\/p\u003e \u003cp\u003e5.4 The Normal Distribution Curve 98\u003c\/p\u003e \u003cp\u003e5.5 Precision of Experimental Data 100\u003c\/p\u003e \u003cp\u003e5.6 Normal Distribution Curve of a Sample 101\u003c\/p\u003e \u003cp\u003e5.7 ChemTech Statistical Calculations 102\u003c\/p\u003e \u003cp\u003e5.8 Student’s Distribution t Test for Confidence Limits 105\u003c\/p\u003e \u003cp\u003e5.9 Tests of Significance 111\u003c\/p\u003e \u003cp\u003e5.10 Treatment of Data Outliers 112\u003c\/p\u003e \u003cp\u003e5.11 Chapter Key Concepts 114\u003c\/p\u003e \u003cp\u003e5.12 Chapter Problems 114\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Plotting and Graphing 116\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction to Graphing 116\u003c\/p\u003e \u003cp\u003e6.2 Graph Construction 116\u003c\/p\u003e \u003cp\u003e6.3 Rectangular Cartesian Coordinate System 117\u003c\/p\u003e \u003cp\u003e6.4 Curve Fitting 117\u003c\/p\u003e \u003cp\u003e6.5 Redrawn Graph Example 117\u003c\/p\u003e \u003cp\u003e6.6 Graphs of Equations 118\u003c\/p\u003e \u003cp\u003e6.7 Least-Squares Method 121\u003c\/p\u003e \u003cp\u003e6.8 Computer-Generated Curves 122\u003c\/p\u003e \u003cp\u003e6.9 Calculating Concentrations 125\u003c\/p\u003e \u003cp\u003e6.10 Nonlinear Curve Fitting 126\u003c\/p\u003e \u003cp\u003e6.11 Chapter Key Concepts 130\u003c\/p\u003e \u003cp\u003e6.12 Chapter Problems 130\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Using Microsoft Excel® in the Laboratory 132\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction to Excel® 132\u003c\/p\u003e \u003cp\u003e7.2 Opening Excel in ChemTech 132\u003c\/p\u003e \u003cp\u003e7.3 The Excel Spreadsheet 132\u003c\/p\u003e \u003cp\u003e7.4 Graphing in Excel 134\u003c\/p\u003e \u003cp\u003e7.5 Complex Charting in Excel 137\u003c\/p\u003e \u003cp\u003e7.6 Statistical Analysis using Excel 139\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Making Laboratory Solutions 151\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 151\u003c\/p\u003e \u003cp\u003e8.2 Laboratory Reagent Fundamentals 151\u003c\/p\u003e \u003cp\u003e8.3 The Periodic Table 151\u003c\/p\u003e \u003cp\u003e8.4 Calculating Formula Weights 152\u003c\/p\u003e \u003cp\u003e8.5 Calculating The Mole 152\u003c\/p\u003e \u003cp\u003e8.6 Molecular Weight Calculator 152\u003c\/p\u003e \u003cp\u003e8.7 Expressing Concentration 153\u003c\/p\u003e \u003cp\u003e8.8 The Parts PER (PP) Notation 157\u003c\/p\u003e \u003cp\u003e8.9 Computer-Based Solution Calculations 157\u003c\/p\u003e \u003cp\u003e8.10 Reactions in Solution 161\u003c\/p\u003e \u003cp\u003e8.11 Chapter Key Concepts 161\u003c\/p\u003e \u003cp\u003e8.12 Chapter Problems 162\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Acid–Base Theory and Buffer Solutions 163\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 163\u003c\/p\u003e \u003cp\u003e9.2 Acids and Bases in Everyday Life 163\u003c\/p\u003e \u003cp\u003e9.3 The Litmus Test 163\u003c\/p\u003e \u003cp\u003e9.4 Early Acid–Base Descriptions 164\u003c\/p\u003e \u003cp\u003e9.5 Brǿnsted–Lowry Definition 164\u003c\/p\u003e \u003cp\u003e9.6 The Equilibrium Constant 165\u003c\/p\u003e \u003cp\u003e9.7 The Acid Ionization Constant 165\u003c\/p\u003e \u003cp\u003e9.8 Calculating the Hydrogen Ion Concentration 165\u003c\/p\u003e \u003cp\u003e9.9 The Base Ionization Constant 167\u003c\/p\u003e \u003cp\u003e9.10 Ion Product for Water 168\u003c\/p\u003e \u003cp\u003e9.11 The Solubility Product Constant (K SP) 168\u003c\/p\u003e \u003cp\u003e9.12 The pH of a Solution 170\u003c\/p\u003e \u003cp\u003e9.13 Measuring the pH 171\u003c\/p\u003e \u003cp\u003e9.14 Buffered Solutions—Description and Preparing 172\u003c\/p\u003e \u003cp\u003e9.15 ChemTech Buffer Solution Calculator 174\u003c\/p\u003e \u003cp\u003e9.16 Chapter Key Concepts 175\u003c\/p\u003e \u003cp\u003e9.17 Chapter Problems 175\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Titration—A Volumetric Method of Analysis 178\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 178\u003c\/p\u003e \u003cp\u003e10.2 Reacting Ratios 178\u003c\/p\u003e \u003cp\u003e10.3 The Equivalence Point 179\u003c\/p\u003e \u003cp\u003e10.4 Useful Relationships for Calculations 179\u003c\/p\u003e \u003cp\u003e10.5 Deriving the Titration Equation 179\u003c\/p\u003e \u003cp\u003e10.6 Titrations in ChemTech 180\u003c\/p\u003e \u003cp\u003e10.7 Acid\/Base Titration Endpoint (Equivalence Point) 181\u003c\/p\u003e \u003cp\u003e10.8 Acid\/Base Titration Midpoint 182\u003c\/p\u003e \u003cp\u003e10.9 Acid\/Base Titration Indicators 182\u003c\/p\u003e \u003cp\u003e10.10 Titrations Using Normal Solutions 184\u003c\/p\u003e \u003cp\u003e10.11 Polyprotic Acid Titration 184\u003c\/p\u003e \u003cp\u003e10.12 ChemTech Calculation of Normal Titrations 185\u003c\/p\u003e \u003cp\u003e10.13 Performing a Titration 185\u003c\/p\u003e \u003cp\u003e10.14 Primary Standards 186\u003c\/p\u003e \u003cp\u003e10.15 Standardization of Sodium Hydroxide 187\u003c\/p\u003e \u003cp\u003e10.16 Conductometric Titrations (Nonaqueous Solutions) 189\u003c\/p\u003e \u003cp\u003e10.17 Precipitation Titration (MOHR Method for Halides) 190\u003c\/p\u003e \u003cp\u003e10.18 Complex Formation with Back Titration (Volhard Method for Anions) 191\u003c\/p\u003e \u003cp\u003e10.19 Chapter Key Concepts 196\u003c\/p\u003e \u003cp\u003e10.20 Chapter Problems 197\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Oxidation–Reduction (Redox) Reactions 199\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 199\u003c\/p\u003e \u003cp\u003e11.2 Oxidation and Reduction 199\u003c\/p\u003e \u003cp\u003e11.3 The Volt 200\u003c\/p\u003e \u003cp\u003e11.4 The Electrochemical Cell 200\u003c\/p\u003e \u003cp\u003e11.5 Redox Reaction Conventions 200\u003c\/p\u003e \u003cp\u003e11.6 The Nernst Equation 202\u003c\/p\u003e \u003cp\u003e11.7 Determining Redox Titration Endpoints 203\u003c\/p\u003e \u003cp\u003e11.8 Potentiometric Titrations 204\u003c\/p\u003e \u003cp\u003e11.9 Visual Indicators used in Redox Titrations 206\u003c\/p\u003e \u003cp\u003e11.10 Pretitration Oxidation–Reduction 207\u003c\/p\u003e \u003cp\u003e11.11 Ion-Selective Electrodes 207\u003c\/p\u003e \u003cp\u003e11.12 Chapter Key Concepts 207\u003c\/p\u003e \u003cp\u003e11.13 Chapter Problems 209\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Laboratory Information Management System (LIMS) 211\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 211\u003c\/p\u003e \u003cp\u003e12.2 Lims Main Menu 211\u003c\/p\u003e \u003cp\u003e12.3 Logging in Samples 213\u003c\/p\u003e \u003cp\u003e12.4 Entering Test Results 213\u003c\/p\u003e \u003cp\u003e12.5 Add or Delete Tests 214\u003c\/p\u003e \u003cp\u003e12.6 Calculations and Curves 214\u003c\/p\u003e \u003cp\u003e12.7 Search Wizards 215\u003c\/p\u003e \u003cp\u003e12.8 Approving Samples 217\u003c\/p\u003e \u003cp\u003e12.9 Printing Sample Reports 217\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Ultraviolet and Visible (UV\/VIS) Spectroscopy 227\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction to Spectroscopy in the Analytical Laboratory 227\u003c\/p\u003e \u003cp\u003e13.2 The Electromagnetic Spectrum 227\u003c\/p\u003e \u003cp\u003e13.3 Ultraviolet\/Visible (UV\/VIS) Spectroscopy 227\u003c\/p\u003e \u003cp\u003e13.4 UV\/Visible Spectrophotometers 236\u003c\/p\u003e \u003cp\u003e13.5 Special Topic (Example)—Spectrophotometric Study of Dye Compounds 237\u003c\/p\u003e \u003cp\u003e13.6 Chapter Key Concepts 240\u003c\/p\u003e \u003cp\u003e13.7 Chapter Problems 242\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Fluorescence Optical Emission Spectroscopy 244\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction to Fluorescence 244\u003c\/p\u003e \u003cp\u003e14.2 Fluorescence and Phosphorescence Theory 245\u003c\/p\u003e \u003cp\u003e14.3 Phosphorescence 246\u003c\/p\u003e \u003cp\u003e14.4 Excitation and Emission Spectra 247\u003c\/p\u003e \u003cp\u003e14.5 Rate Constants 247\u003c\/p\u003e \u003cp\u003e14.6 Quantum Yield Rate Constants 248\u003c\/p\u003e \u003cp\u003e14.7 Decay Lifetimes 248\u003c\/p\u003e \u003cp\u003e14.8 Factors Affecting Fluorescence 249\u003c\/p\u003e \u003cp\u003e14.9 Quantitative Analysis and Beer–Lambert Law 252\u003c\/p\u003e \u003cp\u003e14.10 Quenching of Fluorescence 253\u003c\/p\u003e \u003cp\u003e14.11 Fluorometric Instrumentation 253\u003c\/p\u003e \u003cp\u003e14.12 Special Topic—Fluorescence Study of Dye-A007 Complexes 259\u003c\/p\u003e \u003cp\u003e14.13 Chapter Key Concepts 261\u003c\/p\u003e \u003cp\u003e14.14 Chapter Problems 262\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Fourier Transform Infrared (FTIR) Spectroscopy 264\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15.1 Introduction 264\u003c\/p\u003e \u003cp\u003e15.2 Basic IR Instrument Design 264\u003c\/p\u003e \u003cp\u003e15.3 The Infrared Spectrum and Molecular Assignment 266\u003c\/p\u003e \u003cp\u003e15.4 FTIR Table Band Assignments 267\u003c\/p\u003e \u003cp\u003e15.5 FTIR Spectrum Example I 273\u003c\/p\u003e \u003cp\u003e15.6 FTIR Spectrum Example II 273\u003c\/p\u003e \u003cp\u003e15.7 FTIR Inorganic Compound Analysis 274\u003c\/p\u003e \u003cp\u003e15.8 Chapter Key Concepts 274\u003c\/p\u003e \u003cp\u003e15.9 Chapter Problems 276\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Nuclear Magnetic Resonance (NMR) Spectroscopy 280\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e16.1 Introduction 280\u003c\/p\u003e \u003cp\u003e16.2 Frequency and Magnetic Field Strength 280\u003c\/p\u003e \u003cp\u003e16.3 Continuous-Wave NMR 281\u003c\/p\u003e \u003cp\u003e16.4 The NMR Sample Probe 282\u003c\/p\u003e \u003cp\u003e16.5 Pulsed-Field Fourier Transform NMR 282\u003c\/p\u003e \u003cp\u003e16.6 Proton NMR Spectra Environmental Effects 284\u003c\/p\u003e \u003cp\u003e16.7 Carbon-13 NMR 286\u003c\/p\u003e \u003cp\u003e16.8 Special Topic—NMR Characterization of Cholesteryl Phosphate 290\u003c\/p\u003e \u003cp\u003e16.9 Chapter Key Concepts 295\u003c\/p\u003e \u003cp\u003e16.10 Chapter Problems 296\u003c\/p\u003e \u003cp\u003eReferences 297\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Atomic Absorption Spectroscopy (AAS) 298\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e17.1 Introduction 298\u003c\/p\u003e \u003cp\u003e17.2 Atomic Absorption and Emission Process 298\u003c\/p\u003e \u003cp\u003e17.3 Atomic Absorption and Emission Source 299\u003c\/p\u003e \u003cp\u003e17.4 Source Gases and Flames 299\u003c\/p\u003e \u003cp\u003e17.5 Block Diagram of AAS Instrumentation 299\u003c\/p\u003e \u003cp\u003e17.6 The Light Source 301\u003c\/p\u003e \u003cp\u003e17.7 Interferences in AAS 302\u003c\/p\u003e \u003cp\u003e17.8 Electrothermal Atomization—Graphite Furnace 302\u003c\/p\u003e \u003cp\u003e17.9 Instrumentation 303\u003c\/p\u003e \u003cp\u003e17.10 Flame Atomic Absorption Analytical Methods 304\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18 Atomic Emission Spectroscopy 306\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e18.1 Introduction 306\u003c\/p\u003e \u003cp\u003e18.2 Elements in Periodic Table 306\u003c\/p\u003e \u003cp\u003e18.3 The Plasma Torch 306\u003c\/p\u003e \u003cp\u003e18.4 Sample Types 307\u003c\/p\u003e \u003cp\u003e18.5 Sample Introduction 308\u003c\/p\u003e \u003cp\u003e18.6 ICP-OES Instrumentation 308\u003c\/p\u003e \u003cp\u003e18.7 ICP-OES Environmental Application Example 313\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 Atomic Mass Spectrometry 328\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e19.1 Introduction 328\u003c\/p\u003e \u003cp\u003e19.2 Low-Resolution ICP-MS 328\u003c\/p\u003e \u003cp\u003e19.3 High-Resolution ICP-MS 331\u003c\/p\u003e \u003cp\u003e\u003cb\u003e20 X-Ray Fluorescence (XRF) and X-Ray Diffraction (XRD) 336\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e20.1 X-Ray Fluorescence Introduction 336\u003c\/p\u003e \u003cp\u003e20.2 X-Ray Fluorescence Theory 336\u003c\/p\u003e \u003cp\u003e20.3 Energy-Dispersive X-Ray Fluorescence (EDXRF) 337\u003c\/p\u003e \u003cp\u003e20.4 Wavelength Dispersive X-Ray Fluorescence (WDXRF) 340\u003c\/p\u003e \u003cp\u003e20.5 Applications of XRF 344\u003c\/p\u003e \u003cp\u003e20.6 X-Ray Diffraction (XRD) 345\u003c\/p\u003e \u003cp\u003e\u003cb\u003e21 Chromatography—Introduction and Theory 354\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e21.1 Preface 354\u003c\/p\u003e \u003cp\u003e21.2 Introduction to Chromatography 354\u003c\/p\u003e \u003cp\u003e21.3 Theory of Chromatography 354\u003c\/p\u003e \u003cp\u003e21.4 The Theoretical Plate Number N 358\u003c\/p\u003e \u003cp\u003e21.5 Resolution Rs 359\u003c\/p\u003e \u003cp\u003e21.6 Rate Theory Versus Plate Theory 360\u003c\/p\u003e \u003cp\u003e21.7 Retention Factor k′ 365\u003c\/p\u003e \u003cp\u003eReferences 365\u003c\/p\u003e \u003cp\u003e\u003cb\u003e22 High-Performance Liquid Chromatography (HPLC) 366\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e22.1 HPLC Background 366\u003c\/p\u003e \u003cp\u003e22.2 Design and Components of HPLC 366\u003c\/p\u003e \u003cp\u003e\u003cb\u003e23 Solid-Phase Extraction 383\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e23.1 Introduction 383\u003c\/p\u003e \u003cp\u003e23.2 Disposable SPE Columns 383\u003c\/p\u003e \u003cp\u003e23.3 SPE Vacuum Manifold 383\u003c\/p\u003e \u003cp\u003e23.4 SPE Procedural Bulletin 383\u003c\/p\u003e \u003cp\u003e\u003cb\u003e24 Plane Chromatography: Paper and Thin-Layer Chromatography 397\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e24.1 Plane Chromatography 397\u003c\/p\u003e \u003cp\u003e24.2 Thin-Layer Chromatography 398\u003c\/p\u003e \u003cp\u003e24.3 Retardation Factor (R F) In TLC 399\u003c\/p\u003e \u003cp\u003e24.4 Plate Heights (H) and Counts (N) In TLC 400\u003c\/p\u003e \u003cp\u003e24.5 Retention Factor In TLC 401\u003c\/p\u003e \u003cp\u003e\u003cb\u003e25 Gas-Liquid Chromatography 402\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e25.1 Introduction 402\u003c\/p\u003e \u003cp\u003e25.2 Theory and Principle of GC 402\u003c\/p\u003e \u003cp\u003e25.3 Mobile-Phase Carrier Gasses in GC 404\u003c\/p\u003e \u003cp\u003e25.4 Columns and Stationary Phases 405\u003c\/p\u003e \u003cp\u003e25.5 Gas Chromatograph Injection Port 407\u003c\/p\u003e \u003cp\u003e25.6 The GC Oven 415\u003c\/p\u003e \u003cp\u003e25.7 GC Programming and Control 418\u003c\/p\u003e \u003cp\u003e25.8 GC Detectors 419\u003c\/p\u003e \u003cp\u003e\u003cb\u003e26 Gas Chromatography–Mass Spectrometry (GC–MS) 422\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e26.1 Introduction 422\u003c\/p\u003e \u003cp\u003e26.2 Electron Ionization (EI) 422\u003c\/p\u003e \u003cp\u003e26.3 Electron Ionization (EI)\/OE Processes 423\u003c\/p\u003e \u003cp\u003e26.4 Oleamide Fragmentation Pathways: OE M +⋅ by Gas Chromatography\/Electron Ionization Mass Spectrometry 426\u003c\/p\u003e \u003cp\u003e26.5 Oleamide Fragmentation Pathways: EE [M+H] + by ESI\/ION Trap Mass Spectrometry 427\u003c\/p\u003e \u003cp\u003e26.6 Quantitative Analysis by GC\/EI–MS 430\u003c\/p\u003e \u003cp\u003e26.7 Chapter Problems 432\u003c\/p\u003e \u003cp\u003eReferences 434\u003c\/p\u003e \u003cp\u003e\u003cb\u003e27 Special Topics: Strong Cation Exchange Chromatography and Capillary Electrophoresis 436\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e27.1 Introduction 436\u003c\/p\u003e \u003cp\u003e27.2 Strong Ion Exchange HPLC 436\u003c\/p\u003e \u003cp\u003e27.3 Cze 436\u003c\/p\u003e \u003cp\u003e27.4 Binding Constants by Cation Exchange and CZE 437\u003c\/p\u003e \u003cp\u003e27.5 Comparison of Methods 446\u003c\/p\u003e \u003cp\u003e27.6 Conclusions 449\u003c\/p\u003e \u003cp\u003eReferences 449\u003c\/p\u003e \u003cp\u003e\u003cb\u003e28 Mass Spectrometry 450\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e28.1 Definition and Description of Mass Spectrometry 450\u003c\/p\u003e \u003cp\u003e28.2 Basic Design of Mass Analyzer Instrumentation 450\u003c\/p\u003e \u003cp\u003e28.3 Mass Spectrometry of Protein Metabolite and Lipid Biomolecules 452\u003c\/p\u003e \u003cp\u003e28.4 Fundamental Studies of Biological Compound Interactions 456\u003c\/p\u003e \u003cp\u003e28.5 Mass-to-Charge (m\/z) Ratio: How the Mass Spectrometer Separates Ions 457\u003c\/p\u003e \u003cp\u003e28.6 Exact Mass Versus Nominal Mass 459\u003c\/p\u003e \u003cp\u003e28.7 Mass Accuracy and Resolution 460\u003c\/p\u003e \u003cp\u003e28.8 High-Resolution Mass Measurements 462\u003c\/p\u003e \u003cp\u003e28.9 Rings Plus Double Bonds (r+db) 464\u003c\/p\u003e \u003cp\u003e28.10 The Nitrogen Rule in Mass Spectrometry 465\u003c\/p\u003e \u003cp\u003e28.11 Chapter Problems 466\u003c\/p\u003e \u003cp\u003eReferences 466\u003c\/p\u003e \u003cp\u003e\u003cb\u003e29 Ionization in Mass Spectrometry 468\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e29.1 Ionization Techniques and Sources 468\u003c\/p\u003e \u003cp\u003e29.2 Chemical Ionization (CI) 468\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e29.3 Atmospheric Pressure Chemical Ionization (APCI) 472\u003c\/p\u003e \u003cp\u003e29.4 Electrospray Ionization (ESI) 474\u003c\/p\u003e \u003cp\u003e29.5 Nanoelectrospray Ionization (NANO-ESI) 476\u003c\/p\u003e \u003cp\u003e29.6 Atmospheric Pressure Photoionization (APPI) 479\u003c\/p\u003e \u003cp\u003e29.7 Matrix-Assisted Laser Desorption Ionization (MALDI) 484\u003c\/p\u003e \u003cp\u003e29.8 Fab 486\u003c\/p\u003e \u003cp\u003e29.9 Chapter Problems 490\u003c\/p\u003e \u003cp\u003eReferences 490\u003c\/p\u003e \u003cp\u003e\u003cb\u003e30 Mass Analyzers in Mass Spectrometry 492\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e30.1 Mass Analyzers 492\u003c\/p\u003e \u003cp\u003e30.2 Magnetic and Electric Sector Mass Analyzer 492\u003c\/p\u003e \u003cp\u003e30.3 Time-of-Flight Mass Analyzer (TOF\/MS) 496\u003c\/p\u003e \u003cp\u003e30.4 Time-of-Flight\/Time-of-Flight Mass Spectrometer (TOF–TOF\/MS) 498\u003c\/p\u003e \u003cp\u003e30.5 Quadrupole Mass Filter 500\u003c\/p\u003e \u003cp\u003e30.6 Triple Quadrupole Mass Spectrometer (QQQ\/MS) 503\u003c\/p\u003e \u003cp\u003e30.7 Three-Dimensional Quadrupole Ion Trap Mass Spectrometer (QIT\/MS) 504\u003c\/p\u003e \u003cp\u003e30.8 Linear Quadrupole Ion Trap Mass Spectrometer (LTQ\/MS) 506\u003c\/p\u003e \u003cp\u003e30.9 Quadrupole Time-of-Flight Mass Spectrometer (Q-TOF\/MS) 508\u003c\/p\u003e \u003cp\u003e30.10 Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (fticr\/ms) 509\u003c\/p\u003e \u003cp\u003e30.11 Linear Quadrupole Ion Trap Fourier Transform Mass Spectrometer (ltq–ft\/ms) 517\u003c\/p\u003e \u003cp\u003e30.12 Linear Quadrupole Ion Trap Orbitrap Mass Spectrometer (LTQ–Orbitrap\/MS) 519\u003c\/p\u003e \u003cp\u003e30.13 Chapter Problems 527\u003c\/p\u003e \u003cp\u003eReferences 528\u003c\/p\u003e \u003cp\u003e\u003cb\u003e31 Biomolecule Spectral Interpretation: Small Molecules 529\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e31.1 Introduction 529\u003c\/p\u003e \u003cp\u003e31.2 Ionization Efficiency of Lipids 529\u003c\/p\u003e \u003cp\u003e31.3 Fatty Acids 530\u003c\/p\u003e \u003cp\u003e31.4 Wax Esters 538\u003c\/p\u003e \u003cp\u003e31.5 Sterols 542\u003c\/p\u003e \u003cp\u003e31.6 Acylglycerols 547\u003c\/p\u003e \u003cp\u003e31.7 Esi-Mass Spectrometry of Phosphorylated Lipids 550\u003c\/p\u003e \u003cp\u003e31.8 Chapter Problems 556\u003c\/p\u003e \u003cp\u003eReferences 557\u003c\/p\u003e \u003cp\u003e\u003cb\u003e32 Macromolecule Analysis 559\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e32.1 Introduction 559\u003c\/p\u003e \u003cp\u003e32.2 Carbohydrates 559\u003c\/p\u003e \u003cp\u003e32.3 Nucleic Acids 565\u003c\/p\u003e \u003cp\u003e32.4 Chapter Problems 576\u003c\/p\u003e \u003cp\u003eReferences 576\u003c\/p\u003e \u003cp\u003e\u003cb\u003e33 Biomolecule Spectral Interpretation: Proteins 577\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e33.1 Introduction to Proteomics 577\u003c\/p\u003e \u003cp\u003e33.2 Protein Structure and Chemistry 577\u003c\/p\u003e \u003cp\u003e33.3 Bottom-Up Proteomics: Mass Spectrometry of Peptides 578\u003c\/p\u003e \u003cp\u003e33.4 Top-Down Proteomics: Mass Spectrometry of Intact Proteins 588\u003c\/p\u003e \u003cp\u003e33.5 PTM of Proteins 592\u003c\/p\u003e \u003cp\u003e33.6 Systems Biology and Bioinformatics 610\u003c\/p\u003e \u003cp\u003e33.7 Chapter Problems 614\u003c\/p\u003e \u003cp\u003eReferences 616\u003c\/p\u003e \u003cp\u003eAppendix I: Chapter Problem Answers 619\u003c\/p\u003e \u003cp\u003eAppendix II: Atomic Weights and Isotopic Compositions 625\u003c\/p\u003e \u003cp\u003eAppendix III: Fundamental Physical Constants 629\u003c\/p\u003e \u003cp\u003eAppendix IV: Redox Half-Reactions 630\u003c\/p\u003e \u003cp\u003eAppendix V: Periodic Table of Elements 633\u003c\/p\u003e \u003cp\u003eAppendix VI: Installing and Running Programs 635\u003c\/p\u003e \u003cp\u003eIndex 637\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eBryan M. Ham, PhD,\u003c\/b\u003e has worked in analytical chemistry laboratories for more than 25 years. He is currently working for the Department of Homeland Security at the U.S. Customs and Border Protection Office of Trade Headquarters in Washington D.C. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eAihui MaHam, PhD,\u003c\/b\u003e is an expert in nano-materials including the synthesis and characterization of chemical and biological nano-sensors. She is currently working for the Department of Homeland Security at the U.S. Customs and Border Protection Office of Trade Headquarters in Washington D.C.   \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetailed reference covering all aspects of working in laboratories, including safety, fundamentals of analytical techniques, lab instrumentation, and more\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eA comprehensive study of analytical chemistry as it pertains to the laboratory analyst and chemist, \u003ci\u003eAnalytical Chemistry\u003c\/i\u003e begins with an introduction to the laboratory environment, including safety, glassware, common apparatuses, and lab basics, and continues on to guide readers through the fundamentals of analytical techniques, such as spectroscopy and chromatography, and introduce examples of laboratory programs, such as Laboratory Information Management Systems (LIMS). \u003c\/p\u003e\u003cp\u003eThis newly updated and revised Second Edition of \u003ci\u003eAnalytical Chemistry\u003c\/i\u003e offers expanded chapters with new figures and the latest developments in the field. \u003c\/p\u003e\u003cp\u003eIncluded alongside this new edition is an updated companion teaching, reference, and toolkit program called ChemTech. Conveniently available via either app or browser, the ChemTech program contains exercises that highlight and review topics covered in the book and features useful calculators and programs, including solution makers, graphing tools, and more. To aid in reader comprehension, the program also includes an interactive periodic table and chapter summaries.\t \u003c\/p\u003e\u003cp\u003eWritten by two highly qualified authors with significant experience in both practice and academia, \u003ci\u003eAnalytical Chemistry\u003c\/i\u003e covers sample topics such as: \u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eBasic mathematics in the laboratory, including different units, the metric system, significant figures, scientific calculators, and ChemTech conversion tools\u003c\/li\u003e \u003cli\u003eAnalytical data treatment, including errors in the laboratory, precision versus accuracy, normal distribution curves, and determining errors in methodology\u003c\/li\u003e \u003cli\u003ePlotting and graphing, including graph construction, curve fitting, graphs of specific equations, least-squares method, and computer-generated curves\u003c\/li\u003e \u003cli\u003eUltraviolet\/visible (UV\/Vis) spectroscopy, including wave and particle theory of light, light absorption transitions, the color wheel, and pigments\u003c\/li\u003e\n\u003c\/ul\u003e \u003cp\u003eWith complete coverage of the practical aspects of analytical chemistry, \u003ci\u003eAnalytical Chemistry\u003c\/i\u003e prepares students for a rewarding career as a chemist or a laboratory technician. Thanks to ChemTech integration, the book is also a useful and accessible reference for the established chemist or technician already working in the laboratory.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47988735213797,"sku":"NP9781119894452","price":195.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781119894452.jpg?v=1761781383","url":"https:\/\/k12savings.com\/es\/products\/analytical-chemistry-isbn-9781119894452","provider":"K12savings","version":"1.0","type":"link"}