Molecular Imprinting

Molecular imprinting, the polymerization of monomers in the presence of a template molecule which imprints structural information into the resulting polymers, is a scientific field which is rapidly gaining significance for a widening range of applications in biotechnology, biochemistry and pharmaceutical research.

The methods and tools needed to distinguish target molecules from others by means of tailor-made receptors are constantly growing in importance and complexity. This book gives a concise and highly up-to-date overview of the remarkable progress made in this field in the last five years.
The material is comprehensively presented by the authors, giving a thorough insight into fundamentals and applications for researchers in both industry and academy.
"Molecular Imprinting" ist Polymerisation in Gegenwart einer Schablone, die dem resultierenden Polymer eine bestimmte Struktur gibt. Dies kann sowohl für vorteilhafte Eigenschaften des Polymers als auch für die Wiedererkennung der Schablonenstruktur durch das Polymer, dem Schlüssel-Schloß-Prinzip, genutzt werden. Dieses wachsende Forschungsgebiet erfährt zunehmende Bedeutung für Biotechnologie, Biochemie und Pharmazeutika.
Die Methoden und Werkzeuge zur selektiven Erkennung von spezifischen Zielmolekülen durch maßgeschneiderte Rezeptoren werden ständig komplexer und wichtiger. Dieses Buch gibt über den bemerkenswerten Fortschritt der letzten Jahre auf diesem Gebiet einen prägnanten und hochaktuellen Überblick, der sowohl Grundlagen als auch Anwendungen umfaßt und damit gleichermaßen Leser in Forschung und Industrie bedient.
Preface
INTRODUCTION
Importance of Receptor Molecules in Advanced Science and Technology
Naturally Ocurring Receptors
Artificial Receptors
Receptors for Practical Applications
Why is the Molecular Imprinting Method so Promising?
FUNDAMENTALS OF MOLECULAR IMPRINTING
Introduction
General Principle of Molecular Imprinting
Covalent Imprinting and Non-covalent Imprinting
Advantages and Disadvantages of Covalent and Non-Covalent Imprinting
History
EXPERIMENTAL METHODS (I) -
PROCEDURES OF MOLECULAR IMPRINTING
Introduction
Reagents and Experimental Procedures
Covalent Imprinting
Non-covalent Imprinting
"Dummy Molecular Imprinting"
EXPERIMENTAL METHODS (II) -
EVALUATION OF IMPRINTING EFFICIENCY
Chromatographic Experiments
Batchwise Guest-Binding Experiments
Determination of Guest-Binding Constants
SPECTROSCOPIC ANATOMY OF MOLECULAR IMPRINTING REACTIONS
Introduction
Structures of Adducts in the Pre-organization Step
Determination of the Binding Constant K for the Formation of Monomer-Template Adduct
Relationship between K Value and Imprinting Efficiency
Structure of the Guest-Binding Site
FLOW-CHART OF A TYPICAL MOLECULAR IMPRINTING
Introduction
Choice of Agents
Polymerization
Packing of Imprinted Polymers into the HPLC Columns
Quantitative Evaluation of Imprinting Efficiency
APPLICATIONS OF MOLECULARLY IMPRINTED POLYMERS
Introduction
Sensor Applications
Signaling Polymers
Molecularly Imprinted Sorbent Assays
Molecularly Imprinted Membranes
Affinity-based Solid-phase Extraction
Molecularly Imprinted Catalysts
RECENT CHALLENGES AND PROGRESS
Introduction
Molecular Imprinting in Water
Use of Two Kinds of Functional Monomers for Cooperative Recognition
Inorganic Gel as the Matrix for Molecular Imprinting
Artificial Enzyme (Molecular Catalyst) by Molecular Imprinting
Catalytic Antibody Prepared by Using Transition-State Analog
CONCLUSIONS AND PROSPECTS
Index "..a clear and detailed description..of interest to graduate students and researchers.."
IEEE Electrical Insulation Magazine

"...la tercera parte enumera un buen número de aplicaciones...En resumen, la obra es una revisión concisa y didáctica del progreso desarrollado en este campo de la ciencia durante los últimos cinco anos. Por todo ello, el libro aunque desenado inicialmente para graduados, ofrece también un gran interés para los investigadores industriales y con actividad en medois académos.
Revista de Plásticos Modernos

"...written in an easily understandable fashion that requires only a rudimentary knowledge of chemistry. The book is concise but presents many examples and allows the reader to obtain an understanding of this technology and the future implications without considerable time and effort. It is highly recommended for those interested in learning about an emerging technology that has relevance to chemistry (both synthetic and analytical), polymer science and biology as well as libraries covering these areas of science and technology."
Polymer News

"The book is very helpful for advanced-course students and researchers, who want to find an overview in this new methodology and to gain valuable hints for their future work."
Colloid & Polymer Science

"... compact, well-illustrated and excellently written textbook ..."
Cellulose Chemistry and Technology
(ed. by the Romanian Academy)

"Dieses kurze Lehrbuch ist allen interssierten Studierenden zu empfehlen, sei es als Einzellektüre oder als Kurs-begleitendes Buch. Neulinge, die in dieses Forschungsgebiet einsteigen wollen, werden es als Einführung zu schätzen wissen..." Angewandte Chemie

Dr. Makoto Komiyama is Assistant Professor of Chemistry at the University of Tokyo. Among his awards is the Chemical Society of Japan Award for Young Chemists. Dr. Komiyama's research interests include cyclodextrin chemistry, functionalized polymer chemistry, and molecular complex chemistry. He received his BA and PhD degrees in chemistry from the University of Tokyo.

Makoto Komiyama is the author of Molecular Imprinting: From Fundamentals to Applications, published by Wiley.

Takashi Mukawa is the author of Molecular Imprinting: From Fundamentals to Applications, published by Wiley.

Hiroyuki Asanuma is the author of Molecular Imprinting: From Fundamentals to Applications, published by Wiley.

Molecular imprinting, the polymerization of monomers in the presence of a template molecule which imprints structural information into the resulting polymers, is a scientific field which is rapidly gaining significance for a widening range of applications in biotechnology, biochemistry and pharmaceutical research.

The methods and tools needed to distinguished target molecules from others by means of tailor-made receptors are constantly growing in importance and complexity. This book gives a concise and highly up-to-date overview of the remarkable progress made in this field in the last five years.

The material is comprehensively presented by the authors, giving a thorough insight into fundamentals and applications for researchers in both industry and academy.