Culture of Human Tumor Cells

This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. Written in a reader-friendly style, this book is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications are discussed. An extensive listing of vendors for equipment and other cell culture products is also included.Ein neuer Band aus der 'Culture of Specialized Cells'-Reihe.

Leserfreundlich aufgemacht.

Er vermittelt spezifische praktische Details, wie man Medien und Reagenzien sowie Protokolle für Zellisolierung und Zellkultur präpariert.

Logisch aufgebaut und nach spezifischen Tumoren gegliedert.

Farbtafeln demonstrieren anschaulich Immunozytochemie und Fluoreszenz in situ Hybridisierung (FISH).

Darüber hinaus beschreibt das Buch auch umfangreiche Sicherheitsvorkehrungen.

Mit einer Vielzahl nützlicher Tipps.

Mit einem Glossar zu ausgewählten Fachtermini.

Enthält eine umfangreiche Liste mit Bezugsadressen von Ausrüstung und Zellkulturprodukten.

Erläutert medikamentöse Behandlung, Auswahl, Differenzierung, Assays für die Untersuchung maligner Zellen sowie Risiken und Anwendungsmöglichkeiten.

Preface vii

Contributors ix

List of Abbreviations xiii

1. Growth of Human Lung Tumor Cells in Culture 1
Reen Wu

2. Culture of Normal and Malignant Gastric Epithelium 23
Jae-Gahb Park, Ja-Lok Ku, Hee-Sung Kim, So-Yeon Park and MichaelJ.Rutten

3. Establishment of Cell Lines from Colon Carcinoma 67
Robert H. Whitehead

4. Pancreatic Cancer-Derived Cultured Cells: Genetic Alterations and Application t o an Experimental Model of Pancreatic Cancer Metastasis 81
Haruo Iguchi, Kazuhiro Mizumoto, Masaki Shono, Akira Kono and Soichi Takiguchi

5. The Establishment and Characterization of Bladder Cancer Cultures In Vitro 97
Vivian X. Fu, Steven R. Schwarze, Catherine A. Reznikoff and David F. Jarrard

6. Long-term Culture of Normal and Malignant Human Prostate Epithelial Cells 125
Robert K. Bright and Jennifer D. Lewis

7. The Development of Human Ovarian Epithelial Tumor Cell Lines from Solid Tumors and Ascites 145
Anne P.Wilson

8. Culture of Cervical Carcinoma Tumor Cell Lines 179
Peter Stern, Catherine West and Deborah Burt

9. Primary Culture of Human Mammary Tumor Cells 205
Valerie Speirs

10. Myoepithelium: Methods of Culture and Study 221
Sanford H. Barsky and Mary L. Alpaugh

11. Multistage Head and Neck Squamous Cell Carcinoma 261
Kirsten G. Edington, lsabella J. Berry, Margaret O'Prey, Julie E. Burns, Louise J. Clark, Roy Mitchell, Gerry
Robertson, David Soutar, Lesley W. Coggins and E. Kenneth Parkinson

12. Culture of Melanocytes from Normal, Benign, and Malignant Lesions 289
Ruth Halaban

13. Establishment and Culture of Human Leukemia-Lymphoma Cell Lines 319
Hans G. Drexler

14. In Vitro Culture of Malignant Brain Tumors 349
John L. Darling

15. Culture of Human Neuroendocrine Tumor Cells 373
Roswitha Pfragner, Annemarie Behmel, Elisabeth lngolic and Gerhard H. Wirnsberger

Suppliers 405

Index 419

"This book should be used as reference source for culturing different tumor types. It is a cookbook of sorts, but instead of recipes it has protocols." (E-STREAMS, August 2004) R. Ian Freshney gained a B.Sc. (1960) and Ph.D. (1964) from Glasgow University, where he is on the faculty of Medical Oncology as a senior lecturer and an Honorary Senior Research Fellow. Dr. Freshney currently teaches a number of international basic and specialized cell culture courses, and his research interests include human tumor culture. He is the author of Culture of Animal Cells, a Manual of Basic Technique, and has edited numerous other books on specialized cell culture.

Roswitha Pfragner, Ph.D., studied biology at the University of Vienna, Austria and is currently a senior lecturer at the Institute of Pathophysiology. Her research interests include human neuroendocrine tumors, multiple endocrine neoplasia, drug resistance, apoptosis, and genetic events in tumorigenesis.

Human cell culture in experimental cancer research is taking on new importance in the post-genomic era, as new methods for genome-wide analysis allow researchers to analyze the effects of altered genomes on the cell. Roswitha Pfragner and R. Ian Freshney’s critical compilation introduces culture of human tumor cells, from basic principles to detailed protocols, to the seasoned researcher and novice alike.

Chapters are organized by tumors of clinical significance, and offer informative overviews of pathology, preparation, troubleshooting, applications, alternative methods, and a list of suppliers. This wide-ranging text covers culture for lung tumors, gastric cancer, colorectal cancer, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. In addition, Culture of Human Tumor Cells:

• Provides stepwise protocols by recognized experts
• Presents detailed techniques for initiation, propagation, and characterization of tumor cell lines
• Includes extensive listing of vendors for equipment and other cell culture products
• Discusses drug treatment, selection, differentiation, assays for malignant cells, risks, and applications
• Offers safety precautions, helpful tips, and a list of abbreviations

• Provides specific, practical details on how to prepare media and reagents for the isolation of culture protocols

Scientists, research clinicians, students, and technicians in pathology, cancer biology, cell biology, biochemistry, biotechnology, and medicine will find Culture of Human Tumor Cells to be an incomparable resource.