Cell and Tissue Culture for Medical Research

Cell and tissue culture is a technique in which plant or animal cells are grown under controlled conditions in the laboratory. This is then used for the analysis of the cells themselves, the assessment of the cell's response to chemicals, or as a tool to produce cellular-derived protein products.

This book is a collection of fundamental and specific applied procedures in cell and tissue culture which form the basis of the new medical techniques of tissue engineering and gene therapy. It combines both detailed laboratory procedures and informative overviews.
* Provides step-by-step protocols with troubleshooting tips and notes on time considerations.
* Main procedures are supplemented by alternative procedures, background information and references.
* Experimental examples indicate expected results.Die Kultur von Zellen und Geweben gehört zu den klassischen Arbeitsmethoden der Virologie sowie der genetischen, physiologischen und metabolischen Erforschung der Entstehung von Krankheiten. Dieser Band widmet sich sowohl Standardverfahren als auch hochspezialisierten Methoden. Eine gelungene Kombination aus methodischen Erläuterungen und Hintergrundinformationen! (04/00)

Contributors xv

Preface xxiii

Safety xxiv

Chapter 1 Basic Laboratory Set-Up and Procedures 1

Chapter 2 Specialized Techniques 45

Chapter 3 Cell Characterization and Analysis 91

Chapter 4 Cell Line Derivation Methods 147

Chapter 5 Modern Clinical Applications of Cultured Cells 329

Appendix 1: Terminology 425

Appendix 2: Company Addresses 434

Index 439

"it will be of great value to researchers who require practical guidance for application of cell culture, tissue engineering, and gene therapy." (European Jnl of Orthodontics, Vol 23/1, 2001)

"...The book represents good value for the price and presents many insights..." (Cell Biology International, August 2002)

Alan Doyle is the director of the Fountain House Institute. He is a former assistant director for occupational education for a regional school system on Long Island, has served as the federal liaison for the Massachusetts State Education Commissioner, and attended Fordham University and the Harvard Graduate School of Education. Cell and Tissue Culture for Medical Research is a collection of fundamental and specific applied procedures in cell and tissue culture for clinical research including those that form the basis for the new medical techniques of tissue engineering and gene therapy. This book combines detailed laboratory procedures and informative overviews to aid both laboratory work and the implementation of new lines of investigation, providing:
* Step-by-step protocols with troubleshooting tips;

* Alternative procedures, background reading and references to supplement the main procedures;

* Experimental examples to indicate expected results;

* Quick reference symbols, such as safety icons with warning notes; and,

* Suppliers' details to allow easy access to laboratory products.
Written by a team of international scientists, Cell and Tissue Culture for Medical Research will be of interest to clinical researchers and technicians who use cell and tissue culture to study disease. The book will be particularly valuable to those moving into areas where cell and tissue culture is a new or increasingly important tool in their research.