Biological Applications of Infrared Spectroscopy

Infrared spectroscopy (IR) is a well established analytical technique for the identification of organic molecules. In this first dedicated volume, the theory of IR is described and is then related to various biological systems. Chapters on instrumentation, sample preparation and the interpretation of spectra give the reader practical help in using the technique. A comprehensive applications chapter illustrates the diversity and power of this technique in real systems.Diese Einführung in die Grundlagen der Infrarot-Spektroskopie ist besonders für Biologen und Biochemiker gedacht. Von den theoretischen Grundlagen über experimentelle Methoden und instrumentelle Ausrüstung bis hin zur Interpretation von Spektren biologisch relevanter Spezies (Proteine, Peptide, Lipide, Nucleinsäuren, Membranen) wird das Gebiet umfassend und auch für Nichtspezialisten verständlich behandelt. Instrumentation.

Sampling Methods.

Spectrum Interpretation.

Quantitative Analysis.

Applications.

Self-Assessment Questions and Responses.

Units of Measurement.

Index.

Aus dem Inhalt:
Introduction: Electromagnetic Radiation;
Distribution of Lifetimes of Energy States;
Molecular Vibrations; Instrumentation: Representation of Spectra;
Dispersive Spectrometers;
Fourier Transform Spectrometers; Sampling Methods: Transmission methods;
Reflectance Methods;
Microsampling Methods;
Gas Chromatography;
Temperature Studies;
Photoacoustic Spectroscopy; Spectrum Interpretation: Group Frequencies;
Summary of Characteristic Frequencies;
Aldehydes and Ketones;
Complicating Factors;
Interpretation of Unknown Compounds; Quantitative Analysis: Spectrum Manipulation;
Pathlength Calibration;
Determination of Concentration;
Simple Analysis;
Multicomponent Analysis; Applications: Proteins and Peptides;
Lipids;
Biomembranes;
Animal Tissue;
Carbohydrates;
Pharmaceuticals;
Foods;
Plant Material.

Barbara H. Stuart is the author of Biological Applications of Infrared Spectroscopy, published by Wiley. David J. Ando is the author of Biological Applications of Infrared Spectroscopy, published by Wiley.

Biological Applications of Infrared Spectroscopy Infrared spectroscopy (IR) is a well established analytical technique for the identification of organic molecules. However, it is now being used more and more by biologists and biochemists in the analysis of complex biological molecules, such as proteins, lipids and nucleic acids. In this first dedicated volume, which is aimed at the beginner user level, the theory of IR is described and is then related to various biological systems. Chapters on instrumentation, sample preparation and the interpretation of spectra give the reader practical help in using the technique. A comprehensive applications chapter illustrates the diversity and power of this technique in real systems. Analytical Chemistry by Open Learning This series provides a uniquely comprehensive and integrated coverage of analytical chemistry, covering basic concepts, classical methods, instrumental techniques and applications. The learning objectives of each text are clearly identified and the student's understanding of the material is constantly challenged by self-assessment questions with reinforcing or remedial responses. The overall objective of Analytical Chemistry by Open Learning is to enable the student to select and apply appropriate methods and techniques to solve analytical problems, and to interpret the results obtained.