Production of Recombinant Proteins

While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems -- mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example.

With a foreword by Herbert P. Schweizer, Colorado State University, USA:
"As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."Dieser hochaktuelle Band erspart Ihnen zeitaufwendige Recherchen in der Originalliteratur! Forscher an Universitäten und in der Industrie, die nach einer geeigneten Expressionsplattform für ein gewünschtes Protein suchen, werden hier fündig. Enthalten sind außerdem Informationen zur Biologie des Wirtsorganismus, ein umfangreicher methodologischer Abschnitt sowie zahlreiche Beispiele von Proteinen, die mit Hilfe einer zugehörigen Plattform exprimiert wurden. Mit einem Vorwort von Herbert P. Schweizer, Colorado State University, USA. Foreword by Herbert P. Schweizer, Colorado State University, USA

Introduction

Key and Criteria to the Selection of an Expression Platform

BACTERIAL SYSTEMS
Escherichia coli
Pseudomonas fluorescens
Staphylococcus carnosus and other Gram-positive Bacteria

YEAST SYSTEMS
Arxula adeninivorans
Hansenula polymorpha
Pichia pastoris
Yarrowia lipolytica

FILAMENTOUS FUNGI
Aspergillus sojae
Sordaria macrospora

EUKARYOTIC CELLS
Mammalian Cells
Plant Cells

Wide-Range Integrative Expression Vectors for Fungi, Based on Ribosomal DNA Elements
Comparative Fermentation
Recombinant Hepatitis B Vaccines: Disease Characterization and Vaccine Production
Biopharmaceuticals and the Industrial Environment "...this book is an excellent single source on expression systems. The book draws on the expertise of nearly 50 contributors from academia, research establishments and industry...The book is hardbound and well produced."
Biotechnology Advances

"This book is a remarkable source of information concerning the state-of-the-art in designing the most suitable expression system for the product of choice and will become a very valuable reference for researchers in industry as well as research institutes, and also for students due to its review nature. It is a guide for laboratories as well as scientists and graduates in adjacent scientific disciplines. In addition, the supplied information may help to improve the commercialization of these products which is enforcing an interdisciplinary collaboration of scientists of different fields and engineers."
Engineering in Life Sciences
"Fazit: Ein hochinformatives Buch, das jedem interessierten Forscher eine sehr gute Übersicht über mögliche Expressionssysteme zur Verfügung stellt."
Pharmazie in unserer Zeit

"Das Buch umfasst eine Beschreibung der verschiedenen Expressionsplattformen, außerdem Informationen zur Biologie des Wirtsorganismus, ein umfangreicher methodologischer Abschnitt sowie zahlreiche Beispiele von Proteinen, die mit Hilfe einer zugehörigen Plattform exprimiert wurden."
soziale technik
Gerd Gellissen is Senior Scientist at Rhein Biotech GmbH,Dusseldorf and Associate Professor of Microbiology at the Heinrich-Heine University of Dusseldorf.
He has published approx. 130 research papers and co-authored 7 patents. Professor Gellissen is editor of the Wiley-VCH book "Hansenula polymorpha" (2002). While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section - with strains, genetic elements, vectors and special methods, where applicable - as well as examples of proteins expressed with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems - mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B B vaccine chosen as a n industrial example.

With a foreword by Herbert P. Schweizer, Colorado State University, USA: "As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."