Practical Immunology

Practical Immunology is a basic text aimed at immunology students and researchers at all levels who need a comprehensive overview of the methodology of immunology. The rapid and startling innovations in immunology over the past two decades have their root in sound experimental practice and it has always been the aim of this book to educate researchers in the design and performance of complex techniques. It will appeal to students of immunlogy, graduate students embarking on bench science, or specialised immunologists who need to use an immunological technique outside their sphere of expertise.

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• The definitive lab "bench book".
  
• A one stop resource.
  
• Techniques explained from first principles.
  
• Basic forms of apparatus described in detail.
  
• Totally revised with new user friendly layout to aid use in the lab.
  
• Includes useful hints and tips.

Foreword to the first edition.

Acknowledgements.

Isolation And Structure Of Immunoglobulins Monoclonal.

Antibodies: Production, Purification And Enzymatic Fragmentation.

Antibody Interactions With Antigens.

Antibodies As Probes.

Immunoassay Isolation Of Cells Phagocytosies, Complement And Antibody-Dependent Cytotoxicity.

Lymphocyte Structure.

Lymphocyte Function.

The Cytokines.

Immunological Manipulations In Vivo.

Appendices.

Index

"Practical Immunology is an indispensable book ..." University of Louvain, Brussels

"...In summary, anyone who wishes to gain a good knowledge of Immunology can benefit from this book." Biochimica Clinica

Frank C. Hay is Emeritus Professor of Immunology, St George's University of London.

Olwyn M.R. Westwood is a Professor of Medical Education at Brunel University of London. She is a reviewer for a number of medical and healthcare education journals and a Principal Fellow of the Higher Education Academy.

Practical Immunology is an introductory text aimed at immunology students and researchers who need a comprehensive overview of the methodology of immunology. Now in its fourth edition, Practical Immunology will appeal to students of immunology; graduate students embarking on bench science; and specialised immunologists who need to use an immunological technique outside their sphere of expertise.

The rapid and startling innovations in immunology over the past two decades have their root in sound experimental practice. Practical Immunology gives straightforward descriptions of the commonest techniques that are often covered, but not always well explained in current literature. It explains how to perform the basic techniques that form the anatomy of experimental immunology, and includes tips for improving the techniques, troubleshooting and other handy advice. Where possible, the techniques are explained in a way that ensures that the purchase of specialist kits is avoidable. For those who are a little rusty on their basic immunology the book also gives background information, making it a highly useful "one stop shop".

This latest edition has been fully revised to include the basic techniques of molecular biology, with a revised section on cytokines, an expanded section on clinical immunology and new techniques in epitope mapping and the study of cell adhersion molecules.