Patch Clamping

Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells.

Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results.

• This book will explain the basic principles and practical application of patch clamp electrophysiology
• Written in a non-technical style to ensure its broad appeal to novice users
• Takes a practical approach
• This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns

Introduction.

Basic Theoretical Principles.

Requirements.

The Practice of Patch Clamping.

Whole-cell Protocols and Data Analysis.

Single-channel Protocols and Data Analysis.

Further Reading.

Index.

Areles Molleman is the author of Patch Clamping: An Introductory Guide to Patch Clamp Electrophysiology, published by Wiley.

Patch Clamping: An Introductory Guide to Patch Clamp Electrophysiology is a concise introduction to the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the fundamentals of the subject, platforms, equipment and environmental control. This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this important technique, including an overview of membrane biophysics, standard experimental design, data analysis and technical concerns.

Aimed at novice and intermediate researchers within neurobiology laboratories, this text will also be of interest to people new to this technique, in universities and research labs as well as research technicians. A useful reference for undergraduates specialising in neurophysiology, neuropharmacology and applied physiology.

Features:

• A self-contained guide introducing basic principles and practical applications
• A practical approach to the subject and written in an accessible non-technical style
• Extensive coverage of recording modes, analysis of results and numerous handy hints on how to avoid common pitfalls.