Culture of Hematopoietic Cells

Provides scientists with step-by-step methods for easy use in the lab when cultivating and experimenting with blood cells. Covering a range of human and mouse cell types, this laboratory manual includes contributions from more than twenty leading investigators who offer their practical expertise on a wide range of topics. Features detailed instructions for reagent preparation and information on the latest specialized equipment and supplies. Partial table of contents:

PRIMITIVE PROGENITOR ASSAYS.

Cobblestone Area Forming Cell (CAFC) Assay (R. Ploemacher).

Assay for Murine Blast Cell Colonies (N. Katayama & M.Ogawa).

Serum-Deprived Cultures of Primary Hematopoietic Cells (G.Migliaccio & A. Migliaccio).

COLONY-FORMING ASSAYS FOR MALIGNANT CELLS.

Colony-Forming Assays for Acute Myeloid Leukemia (S. Rogers, etal.).

LONG-TERM ASSAYS AND IMMORTALIZATION.

Long-Term Culture of Human Myeloid Cells (H. Sutherland & C.Eaves).

Immortalization of Hematopoietic Cells (C. MacDonald).

PURIFICATION OF PROGENITOR CELLS.

Purification of Murine Granulocyte-Macrophage Progenitor Cells(CFU-GM) Using Counterflow Centrifugal Elutriation (S. Cooper &H. Broxmeyer).

OTHER SYSTEMS AND ASSAYS.

Culture of Human and Mouse Bone Marrow Stromal Cells (S. Perkins& R. Fleischman).

List of Suppliers.

Index.

R. IAN FRESHNEY, PhD, is Senior Research Fellow in the Centre for Oncology and Applied Pharmacolgy at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.